Comments on Kämmerer, et al. (2023) regarding RT-PCR Testing

Authors

  • Fabio Franchi Independent Researcher
  • Jerneja Tomsic Researcher at the City of Hope Comprehensive Cancer Center, Duarte USA

DOI:

https://doi.org/10.56098/ijvtpr.v3i1.81

Keywords:

COVID-19 disease, NAAT, nucleic acid amplification testing, RT-PCR

Abstract

Kämmerer et al., (2023) reviewed the trustworthiness of Reverse Transcription – Polymerase Chain Reaction (RT-PCR) and sought to solve the serious problems that remain challenging its use and the significance of its application for the diagnosis of COVID-19 infections. They proposed certain modifications which they argued will enable more precise and trustworthy diagnostic results. However, this paper has a clear focus on the technical aspect of RT-PCR, which is only one piece of the COVID-19 puzzle, while there are other equally important pieces still missing. In order to establish what could correctly be judged as a true positive diagnosis, one sharply distinct from a false positive diagnosis, it is necessary to consider additional details. We believe their recommendations amount only to a partial solution to the deeper problems their work implicates. We propose to look at the larger perspective: to start from the very beginning, that is the definition of COVID-19 disease. 

Author Biography

  • Fabio Franchi, Independent Researcher

    Former “Dirigente Medico” (Medical Director) in the Infectious Disease Ward, Trieste, Italy; who specialized in “Infectious Diseases” and in “Hygiene and Preventive Medicine”.

References

Ai, T., Yang, Z., Hou, H., Zhan, C., Chen, C., Lv, W., Tao, Q., Sun, Z., & Xia, L. (2020). Correlation of Chest CT and RT-PCR Testing in Coronavirus Disease 2019 (COVID-19) in China: A Report of 1014 Cases. Radiology Feb 26:200642. https://doi.org/10.1148/radiol.2020200642

CDC (2021). Coronavirus Disease 2019 (COVID-19). Case Definition https://ndc.services.cdc.gov/case-definitions/coronavirus-disease-2019-2021/

ECDC (2020) Case definition for COVID-19, as of 3 December 2020. https://www.ecdc.europa.eu/en/covid-19/surveillance/case-definition

Kämmerer, U., Pekova, S., Klement, R., Louwen, R., Borger, P., & Steger, K. (2023). RT-PCR test targeting the conserved 5’-UTR of SARS-CoV-2 overcomes shortcomings of the first WHO-recommended RT-PCR test. International Journal of Vaccine Theory, Practice, and Research, 3(1), 818-846. https://doi.org/10.56098/ijvtpr.v3i1.71

Killingley, B., Mann, A.J., Kalinova, M., Boyers, A., Goonawardane, N., Zhou, J., Lindsell, K., Hare, S.S., Brown, J., Frise, R., Smith, E., Hopkins, C., … Chiu C. (2022). Safety, tolerability and viral kinetics during SARS-CoV-2 human challenge in young adults. Nature Medicine, 28(5):1031-1041. https://doi.org/10.1038/s41591-022-01780-9

Lu, R., Zhao, X., Li, J., Niu, P., Yang, B., Wu, H., Wang, W., Song, H., Huang, B., Zhu, N., Bi, Y., ... Tan W.. (2020). Genomic characterization and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet 395:565–574. https://doi.org/10.1016/S0140-6736(20)30251-8

Polack, F.P., Thomas,S.J., Kitchin, N., Absalon, J., Gurtman, A., Lockhart, S., Perez, J.L., Marc, G.P., Moreira, E.D., Zerbini, C., Bailey, R., Swanson, K.A., et al., for the C4591001 Clinical Trial Group (2020). Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine pneumonia in China. New England Journal of Medicine 382:727 383:2603-15. https://doi.org/10.1056/NEJMoa2034577

Ren, L.L., Wang, Y.M., Wu, Z.Q., Xiang, Z.C., Guo, L., Xu, T., Jiang, Y.-Z., Xiong, Y., Li, Y.-J., Li, X.-W., Li, H., Fan, G.-H., Gu, X.-Y., … Wang, J.-W. (2020). Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study. Chinese Medical Journal 133:1015–1024. https://doi.org/10.1097/CM9.0000000000000722

Struyf, T., Deeks, J.J., Dinnes, J., Takwoingi, Y., Davenport, C., Leeflang, M.M.G., Spijker R., Hooft, L., Emperador, D., Dittrich, S., Domen, J., Horn, S. R. A., Ann Van den Bruel, A., Cochrane COVID-19 Diagnostic Test Accuracy Group. (2020). Signs and symptoms to determine if a patient presenting in primary care or hospital outpatient settings has COVID-19 disease. Cochrane Database Systematic Reviews 7:CD013665. https://doi.org/10.1002/14651858.CD013665

WHO (2020) COVID case definition (July 22, 2020). https://www.who.int/publications/i/item/WHO-2019-nCoV-Surveillance_Case_Definition-2022.1

Zhu, N., Zhang, D., Wang, W., Li, X., Yang, B., Song, J. Zhao, X., Huang, B., Shi, W., Roujian Lu, R., Niu, P., ... Tan, W., for the China Novel Coronavirus Investigating and Research Team. (2020). A novel coronavirus from patients with pneumonia in China. New England Journal of Medicine 382:727-733. https://doi.org/10.1056/NEJMoa2001017

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Published

2023-06-10

How to Cite

Comments on Kämmerer, et al. (2023) regarding RT-PCR Testing. (2023). International Journal of Vaccine Theory, Practice, and Research , 3(1), 846.1-846.4. https://doi.org/10.56098/ijvtpr.v3i1.81

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